The figure preparation guidelines that follow clarify PLOS Global Public Health standards and requirements, and aim to ensure the integrity and scientific validity of blot/gel data reporting. The original images also provide additional information for readers about background within the experiment and the specificity of reagents used. The underlying data requirement is in place to ensure that the results are reported in a fully transparent manner, and that readers can verify results by reviewing the primary data in its original form. On a Mac, you can view AI files in Preview without any changes.The following requirements apply to any figures and supporting Information files that report blot or gel data. When the EPS specification was written, file extensions in DOS were limited to 3 characters.If you have an AI file you need to view but not edit, there are lots of programs that. File extensions and Macintosh file types: Because the image type for the preview image that may be stored in an EPS file is significant to operating systems and applications, several different extensions and Macintosh file types have been used.Whilst it is not necessary to provide original images at time of initial submission, we will require these files during the peer review process or before a manuscript can be accepted.Please follow these instructions when preparing and submitting blot/gel data files: Since version 4.x catalog maker plugin does not support Windows XP and older.Authors must provide the original, uncropped and minimally adjusted images supporting all blot and gel results reported in an article’s figures and supporting information files. Many MS Office users complained that they can't insert pictures in a Word document.With this plugin you can extract/view ADF, DMS, ADZ, HDF, HDZ, DMP file. Though the issue can also occur in the later versions of Microsoft Word. This is a common issue in MS Word 20.Authors should label each raw blot or gel image to clearly annotate the loading order, identity of experimental samples, method used to capture the image, and to specify which figure panel was generated from that original image. The file should be named ‘S1_raw_images’ and uploaded as a Supporting Information file or deposited at a suitable publicly-available data repository, with the dataset identifier (DOI or other form of persistent identifier) provided in the Data Availability Statement. We do not recommend compiling the images in Powerpoint as resolution can be lost. You could also use a PDF program to build a single PDF compiled from multiple annotated jpeg/tiff image files. Gimp, Photoshop) to compile and annotate the original images and then exporting/saving as a tiff file with LZW compression. We recommend using image software (e.g.
If your PDF size is larger, please use a suitable repository or discuss with the journal staff.If any concerns arise about the availability of the original blot or gel images or compliance with the figure preparation guidelines (see below), the submission will be placed on hold while this is resolved. Please note, there is a 20 MB maximum file size. All labeling and annotation should be performed without obscuring any data or background bands. The background and contrast should not be modified to notably alter the visibility of data, background, or non-specific bands. In accordance with our Policy on Figure Preparation, the images should not be adjusted in any way that could affect the scientific information displayed. Reporting blot and gel resultsAuthors must comply with the following guidelines when preparing figures that report results from blots (including, but not limited to, western blots) and electrophoretic gels: If concerns are noted after publication, journal staff will follow up with authors and correct the literature as needed. If lanes from a single blot/gel image have been rearranged in preparing a figure, image splicing should be clearly denoted by vertical black lines on the figure and the figure legend should provide details of how the figure was made. If data from multiple blot or gel images are needed to illustrate the results, the different images should not be spliced together they should be clearly distinguished as separate panels within the figure and the legend should note that they represent different gels, blots, or exposures. A figure panel should not include composite images of bands originating from different blots, exposures, or gels. Each figure should include all relevant controls, and when relevant, control samples should be run on the same blot or gel as the experimental samples. Avoiding inappropriate image manipulationWe realize that the extent to which figures can be changed as part of normal preparation can pose a dilemma. Additionally, if further analysis is needed, please be aware that we may also require you to supply us with your original photographic images in the original file format and at the resolution at which they were first created. In preparing figures, image files should not be manipulated or adjusted in any way that could lead to misinterpretation of the information present in the original image.Please read our requirements for reporting blot and gel data and uploading original image files. Please also see our guidelines on Preparing Figures from Image Files for further details.The following guidelines aim to help authors prepare high quality figures and avoid common errors that result from inappropriate image manipulation. When adjusting the figure dimensions, be sure the Chain/Lock symbol is closed. To align your figure with the text column of the PDF version of the article, make it no wider than 5.2 inches (13.2 cm).Ensure that individual images meet the pixel dimensions indicated above when preparing and saving figures with slideshow software (like Powerpoint, OpenOffice, or Keynote).Make sure source images are 2,250 pixels wide if you want figures at full page width of 19.05cm. The closer figures match these dimensions, the closer they will meet expectations on publication. EPS files often have missing/corrupted fonts, oversized masks, stray points, and boxes, which can result in errors and poor output.At the height maximum, the figure occupies the whole page and excludes the caption“Dimensions” refers to the dimensions of the entire figure, excluding any white space. If evidence is found of inappropriate manipulation, we reserve the right to ask for original data and, if that is not satisfactory, we may decide not to accept the manuscript, and may also contact the authors' institutions to ask them to assist with investigation.Should I choose TIFF or EPS? TIFF tends to be easier to work with than EPS. Avast mac cleaner activation codeIf you created a 72 dpi line graph and placed it in a 300 dpi TIFF, the graph will look blurred, jagged, or pixilated.Submit files at a size of 10 MB or less. Resolution below 300 results in blurred, jagged or pixelated published figures. Resolution above 600 dpi may lead to resizing of the published figure.The quality of your figures is only as good as the lowest-resolution element present. To improve its resolution, re-create the figure from the beginning. The quality of a low-resolution figure cannot be improved by simply increasing the resolution in graphics software. Go to Image → Properties (or Image → Information) to view image dimensions.Submit figures at the desired dimensions with a resolution no greater than 300-600 dpi. Download microsoft office in mac for free redditSource images created in EPS format need to be compressed (in your compression format of choice) and should be no larger than full page size. A flattened TIFF has a single layer called “background” and has a smaller file size than a TIFF with “Layer 1”. If you elect not to use PACE, follow the instructions below to manually resize TIFF or EPS files. How Come I Can See An Eps File In Word But Not Windows Update All FigureWhen submitting an initial or revised manuscript, embed figure captions within the manuscript text after their first mention, or group together at the end of the manuscript. Read more about submitting captions.If at any point you change the numbering order of your figures, be sure to update all figure citations, captions, and file names accordingly. That information will go into your figure caption in the manuscript. Legends are optional.Label. There are two required elements: figure label and figure title. Format your figure captions.
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